Depth Chart
| Offense | |||
| Position | |||
| Left Tackle | Trent Williams | Shon Coleman | Isaiah Williams |
| Left Guard | Laken Tomlinson | Dakoda Shepley | |
| Center | Alex Mack | Daniel Brunskill | Taybor Pepper |
| Right Guard | Colton McKivitz | ||
| Right Tackle | Mike McGlinchey | Justin Skule | |
| Wide Receiver | Brandon Aiyuk | Travis Benjamin | Austin Proehl |
| Wide Receiver | Deebo Samuel | Richie James | River Cracraft |
| Running Back | Raheem Mostert | Jeff Wilson Jr. | JaMycal Hasty |
| Tight End | George Kittle | Daniel Helm | Ross Dwelley |
| Quarterback | Jimmy Garoppolo | Josh Rosen | Josh Johnson |
| Defense | |||
| Position | |||
| Left Defensive End | Nick Bosa | Alex Barrett | Daeshon Hall |
| Left Defensive Tackle | Javon Kinlaw | Kevin Givens | Darrion Daniels |
| Right Defensive Tackle | D.J. Jones | Kentavius Street | Josiah Coatney |
| Right Defensive End | Arik Armstead | Dee Ford | Corbin Kaufusi |
| Strong Linebacker | Dre Greenlaw | Jonas Griffith | |
| Middle Linebacker | Fred Warner | Azeez Al-Shaair | Nathan Gerry |
| Weak Linebacker | Samson Ebukam | James Lockhart | Demetrius Flannigan-Fowles |
| Left Cornerback | K'Waun Williams | Emmanuel Moseley | Briean Boddy-Calhoun |
| Right Cornerback | Jason Verrett | Dontae Johnson | Mark Fields II |
| Strong Safety | Jaquiski Tartt | Marcell Harris | Obi Melifonwu |
| Free Safety | Jimmie Ward | Tarvarius Moore | Kai Nacua |
| Special Teams | |||
| Position | |||
| Kicker | Robbie Gould | ||
| Kick-Offs | Robbie Gould | ||
| Punter | Mitch Wishnowsky | ||
| Kick Returner | Richie James | ||
| Punt Returner | Richie James | ||
| Holder | Mitch Wishnowsky | ||
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